The relationship between Adenosine Deaminase (ADA) and Pus primarily involves the use of an ADA test on pus samples as a diagnostic tool for certain infections, particularly Tuberculosis (TB). 1. 🔍 ADA Test on Pus Purpose: The ADA Test - Pus measures the level of the Adenosine Deaminase enzyme in a pus sample. Pus is a thick, cloudy fluid containing white blood cells that accumulates at a site of infection. Enzyme Role: ADA is an enzyme involved in purine metabolism. It is crucial for the proliferation and differentiation of lymphocytes (a type of white blood cell), especially T-lymphocytes. Clinical Significance: Elevated ADA levels in pus can indicate an immune response, commonly observed in tuberculous abscesses or other inflammatory processes. The test is used to help diagnose tuberculosis (TB) when it cannot be easily confirmed by other methods, such as acid-fast bacilli (AFB) smear. High ADA activity in fluid/debris, like pus, is highly suggestive of infection with Mycobacterium tuberculosis (TB bacteria). 2. 🧬 ADA Deficiency (ADA-SCID) While the ADA test in pus relates to infection diagnosis, a different and more severe condition is caused by a lack of the ADA enzyme. What it is: Adenosine Deaminase Deficiency is a rare, inherited disorder that results in a severe form of Severe Combined Immunodeficiency (SCID), often called ADA-SCID.