An Alkaline Phosphatase (ALP) Isoenzymes Electrophoresis Serum test is a diagnostic tool used to determine the specific source of elevated ALP levels in the blood. Because ALP is produced by several organs—mainly the liver, bone, intestine, and placenta—a standard test cannot pinpoint which tissue is damaged.

Electrophoresis solves this by using an electric current to separate these distinct forms (isoenzymes) based on their physical properties. Identifying which specific isoenzyme is elevated allows clinicians to distinguish between liver disease (like blocked bile ducts) and bone disorders (like Paget's disease), ensuring a precise diagnosis and targeted treatment plan.