A large buttock mass biopsy (measuring 3 to 6 cm) is a medical procedure performed to investigate a significant, deep-seated tissue growth or lesion in the gluteal region. Because masses of this size carry a higher risk of being neoplastic, cystic, or inflammatory, obtaining an accurate histological diagnosis is vital. During the procedure, a local or general anesthetic is administered, and a core needle or surgical incision is used to extract representative tissue samples from the 3–6 cm mass. The excised tissue is then sent to pathology to determine malignancy, plan precise treatments, and guide surgical management.